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pgl3 basic empty vector  (Addgene inc)


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    Addgene inc pgl3 basic empty vector
    Pgl3 Basic Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+pgl3+basic+plasmids/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pm40373767-543-11-16
    Average 93 stars, based on 51 article reviews
    pgl3 basic empty vector - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Luciferase:

    Article Title: Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor
    Article Snippet: The wild-type form of the 3′UTR region of the ACTA2 promoter containing the CArG box was subcloned into the pGL3 reporter vector (#E1751, Promega, RRID: Addgene_48743). .. For the luciferase assay, the pretreated primary human EcESCs were plated on 24-well plates and co-transfected with 0.5 μg of pGL3-α-SMA-pro-Luc or empty (pGL3-Basic) plasmids together with 0.05 mg of pRL Renilla luciferase reporter vector (#E2241, Promega, RRID: Addgene_44379) using Lipofectamine 3000 according to the manufacturer’s instructions. ..

    Article Title: Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor.
    Article Snippet: The wild-type form of the 3′UTR region of the ACTA2 promoter containing the CArG box was subcloned into the pGL3 reporter vector (#E1751, Promega, RRID: Addgene_48743). .. For the luciferase assay, the pretreated primary human EcESCs were plated on 24-well plates and co-transfected with 0.5 μg of pGL3-α-SMA-pro-Luc or empty (pGL3-Basic) plasmids together with 0.05 mg of pRL Renilla luciferase reporter vector (#E2241, Promega, RRID: Addgene_44379) using Lipofectamine 3000 according to the manufacturer’s instructions. ..



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    Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

    Journal: Genes

    Article Title: Functional Analysis of Promoter Variants in Genes Involved in Sex Steroid Action, DNA Repair and Cell Cycle Control

    doi: 10.3390/genes10030186

    Figure Lengend Snippet: Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

    Article Snippet: Similar experiments were performed with the empty promoterless pGL3-Basic plasmid (Promega) and the SV40-driven Firefly Luciferase pGL3-control plasmid (Promega, Madison, WI, USA) as negative and positive controls, respectively.

    Techniques: Activity Assay, Luciferase, Plasmid Preparation, Clone Assay, Control, Expressing